Custom Antisense Oligonucleotides (ASOs) Synthesis

ASOs bind specifically to their target RNAs and are widely used for drug target screening and design. With advanced molecular biology platforms, CD Formulation offers high-quality, customized, and large-scale ASO synthesis services.

What are Antisense Oligonucleotides (ASOs)?

Antisense oligonucleotides (ASOs) are lab-made strands of nucleotides, usually longer than 15 bases. They work by entering cells and attaching themselves to specific mRNA targets through complementary base pairing. This process, assisted by an enzyme called ribonuclease H1, ultimately leads to the suppression of the genes those mRNAs were meant to express. Upon binding to the target mRNA or precursor mRNA, ASOs regulate RNA function through several distinct mechanisms.

• Inhibition of translation
• Activation of RNase H-mediated degradation
• Alteration of splicing patterns

Explore Our Custom ASOs Synthesis Services

CD Formulation's platform not only customizes standard, unmodified ASOs but also produces complex ASOs that require multiple modifications.

Chemical Modification Services

We possess extensive expertise in chemical modification technology and capabilities, we can enhance the stability and resistance to nuclease degradation of antisense oligonucleotides through thio-modification.

Method Development Services

Our team develops specialized assays for nucleic acid products and conducts quality studies, methodology validation, standard establishment, stability studies, and other services for new nucleic acid products. We can collaboratively establish quality standards for the production of nucleic acid products, as well as the content and scope of quality studies, tailored to customer objectives and in conjunction with assessments of drug efficacy and pharmacology.

Synthesis from small-scale to pilot-scale to large-scale.

We offer production services ranging from microgram to kilogram levels to meet the needs at every stage.

Our Capabilities

General Workflow for ASOs Preparation

Fig.1 Flow chart of ASOs preparation. (CD Formulation)

• Design and Selection of Target Sequences - Identification of ASOs sequences suitable for targeting specific gene sequences.
• Sequence Synthesis - ASOs are synthesized from the designed sequence using chemical synthesis techniques.
• Purification - The removal of impurities from the synthesis process can be achieved through high-performance liquid chromatography (HPLC) or other methods.
• Quality Control - Sequencing, quality analysis, and concentration measurement of the synthesized ASOs are conducted to ensure its accuracy and validity.
• Modification - Chemically modify the ASOs as necessary to improve its stability and specificity.
• Product Delivery - Deliver custom-synthesized ASO to researchers or institutions for specific experiments or applications.

Our Technology Platforms

Production Platform

CD Formulation offers a customized platform for high-throughput synthesis of oligonucleic acids at an internationally advanced level. Our production center is capable of manufacturing a wide range of nucleic acids in single batches, ranging from micrograms to hundreds of grams and even kilograms. This capability meets the diverse needs of scientific research, development, and the screening of new drugs at various stages.

Quality Control Platform

Our quality control platform offers comprehensive process quality control, spanning from sequence design to final product testing, utilizing advanced synthesis technology and precise sequencing analysis. Our platform's automated processes not only enhance productivity but also minimize human error, resulting in highly consistent product quality.

Why Choose Us for Customized ASOs Synthesis?

• Our synthesis process swiftly aligns with researchers' needs, speeding up the R&D cycle.
• Custom synthesis offers flexibility in designing ASO sequences to optimize goal-specific efficacy.
• Chemical modification boosts ASO stability and half-life, enhancing therapeutic efficacy.

Publication Data

CD Formulation offers customized synthetic ASOs services and is dedicated to supporting researchers and pharmaceutical companies in gene editing and therapeutic applications. Please feel free to contact us for assistance with your innovative drug development projects.

How It Works

STEP 1

Submit a service request describing your specific research or development need.

STEP 2

We'll email you to provide your quote and confirm order details if applicable.

STEP 3

Execute the project with real-time communication, and deliver the final report promptly.